SRP19 Polyclonal antibody

SRP19 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16033-1-AP

Synonyms

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Validation Data Gallery

• 

  WB analysis of human liver using 16033-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16033-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16033-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 16033-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16033-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16033-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 16033-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16033-1-AP

  IP result of anti-SRP19 (IP:16033-1-AP, 4ug; Detection:16033-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human stomach using 16033-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16033-1-AP (SRP19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16033-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SRP19 antibody (16033-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue, A549 cells, HeLa cells, K-562 cells, mouse kidney tissue, mouse liver tissue, mouse ovary tissue, Raji cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

16033-1-AP targets SRP19 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRP19 fusion protein Ag8903
• Full Name: signal recognition particle 19kDa
• Calculated Molecular Weight: 144 aa, 16 kDa
• Observed Molecular Weight: 18-25 kDa
• GenBank Accession Number: BC010947
• Gene Symbol: SRP19
• Gene ID (NCBI): 6728
• RRID: AB_11041714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The signal recognition particle (SRP) is one of the few functional small RNP particles. The SRP couples the synthesis of membrane and secretory proteins across or into the endoplasmic reticulum (ER) membrane in eukaryotes, as well as across the bacterial plasma membrane, and chloroplast thylakoid membranes. The mammalian SRP is composed of a 7S (or 7SL) RNA and six different proteins, SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72. All of the components of SRP, including SRP RNA, participate directly in the overall protein targeting process. SRP19 binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19 was shown to significantly enhance SRP54 attachment to helix 8 of 7SL RNA. Binding of SRP19 leads to restructuring of both helix 6 and 8, causing local changes at the SRP54-binding site. This antibody is a rabbit polyclonal antibody raised against full length SRP19 of human origin.

Publications

Species	Application	Title
	WB	Nucleic Acids Res; The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins.; Authors - Joori Park
human	WB	Nucleic Acids Res; SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.; Authors - Anne-Sophie Gribling-Burrer
human	WB,IP,IF	Life Sci Alliance; The nucleolar phase of signal recognition particle assembly; Authors - Amani Issa
human	WB	PLoS One; The CELF1 RNA-Binding Protein Regulates Decay of Signal Recognition Particle mRNAs and Limits Secretion in Mouse Myoblasts.; Authors - Joseph Russo
human	WB	Elife; The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1.; Authors - Diego Acosta-Alvear