SRFBP1 Polyclonal antibody

SRFBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12792-1-AP

Synonyms

BUD22, P49, p49/STRAP, Rlb1, SRFBP1, STRAP

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Validation Data Gallery

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  WB analysis of mouse heart using 12792-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12792-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 12792-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12792-1-AP (SRFBP1 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12792-1-AP targets SRFBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRFBP1 fusion protein Ag3517
• Full Name: serum response factor binding protein 1
• Calculated Molecular Weight: 429 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC031222
• Gene Symbol: SRFBP1
• Gene ID (NCBI): 153443
• RRID: AB_2877882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRFBP1, also named as P49, p49/STRAP and STRAP, is abundantly expressed in heart and skeletal muscle, and at much lower levels in brain and lung. It may be involved in regulating transcriptional activation of cardiac genes during the aging process. SRFBP1 may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human SRFBP1.