FDFT1 Polyclonal antibody

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  WB analysis using 13128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13128-1-AP (FDFT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13128-1-AP

  WB result of FDFT1 antibody (13128-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDFT1 transfected HepG2 cells.

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  IP experiment of COLO 320 using 13128-1-AP

  IP result of anti-FDFT1 (IP:13128-1-AP, 4ug; Detection:13128-1-AP 1:500) with COLO 320 cells lysate 2400ug.

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  IHC staining of human ovary cancer using 13128-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13128-1-AP (FDFT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 13128-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 13128-1-AP (FDFT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

FDFT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13128-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SQS, Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21

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Tested Applications

• Positive WB detected in: HepG2 cells, T-47D cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IHC: See 3 publications below

Product Information

13128-1-AP targets FDFT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FDFT1 fusion protein Ag3743
• Full Name: farnesyl-diphosphate farnesyltransferase 1
• Calculated Molecular Weight: 417 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC029641
• Gene Symbol: FDFT1
• Gene ID (NCBI): 2222
• RRID: AB_2294094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P37268
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Farnesyl-diphosphate farnesyltransferase 1 (FDFT1), also known as SQS, is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).

Publications

Species	Application	Title
human,mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	Cell Metab; Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer.; Authors - Douglas E Biancur ; KD Validated
mouse	WB	Adv Sci (Weinh); Endothelial Cells Mediated by STING Regulate Oligodendrogenesis and Myelination During Brain Development; Authors - Wenwen Wang
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	WB	Cancer Res; Caffeine supplementation and FOXM1 inhibition enhance the antitumor effect of statins in neuroblastoma; Authors - Gia-Buu Tran ; KD Validated
human	WB	Phytomedicine; Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.; Authors - Xing Yue Xu