FDFT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FDFT1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 13128-1-AP

Synonyms

DGPT, ERG9, FDFT1, FPP:FPP farnesyltransferase, SQS, Squalene synthase, SS

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Validation Data Gallery

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  WB analysis of HepG2 using 13128-1-AP

  WB result of FDFT1 antibody (13128-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDFT1 transfected HepG2 cells.

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  IP experiment of COLO 320 using 13128-1-AP

  IP Result of anti-FDFT1 (IP:13128-1-AP, 4ug; Detection:13128-1-AP 1:500) with COLO 320 cells lysate 2400ug.

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  IF Staining of L02 using 13128-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 13128-1-AP (FDFT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: COLO 320 cells
• Positive IF detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 13 publications below
• IHC: See 3 publications below

Product Information

13128-1-AP targets FDFT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FDFT1 fusion protein Ag3743
• Full Name: farnesyl-diphosphate farnesyltransferase 1
• Calculated molecular weight: 417 aa, 48 kDa
• Observed molecular weight: 48 kDa
• GenBank accession number: BC029641
• Gene symbol: FDFT1
• Gene ID (NCBI): 2222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	Cell Metab; Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer.; Authors - Douglas E Biancur ; KD Validated
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	WB	Cancer Res; Caffeine supplementation and FOXM1 inhibition enhance the antitumor effect of statins in neuroblastoma; Authors - Gia-Buu Tran ; KD Validated
human	WB	Phytomedicine; Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.; Authors - Xing Yue Xu
human	WB	Cancer Med; Identification of FDFT1 as a potential biomarker associated with ferroptosis in ccRCC.; Authors - Ruizhen Huang