• Featured Product
  • KD/KO Validated

FDFT1 Polyclonal antibody

FDFT1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13128-1-AP

Synonyms

SQS, Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21



Tested Applications

Positive WB detected inHepG2 cells, T-47D cells
Positive IP detected inCOLO 320 cells
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13128-1-AP targets FDFT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FDFT1 fusion protein Ag3743
Full Name farnesyl-diphosphate farnesyltransferase 1
Calculated Molecular Weight 417 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC029641
Gene Symbol FDFT1
Gene ID (NCBI) 2222
RRIDAB_2294094
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Farnesyl-diphosphate farnesyltransferase 1 (FDFT1), also known as SQS, is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).

Protocols

Product Specific Protocols
WB protocol for FDFT1 antibody 13128-1-APDownload protocol
IF protocol for FDFT1 antibody 13128-1-APDownload protocol
IP protocol for FDFT1 antibody 13128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
mouseWB

Cell Metab

Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer.

Authors - Douglas E Biancur
  • KD Validated
mouseWB

Adv Sci (Weinh)

Endothelial Cells Mediated by STING Regulate Oligodendrogenesis and Myelination During Brain Development

Authors - Wenwen Wang
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
humanWB

Cancer Res

Caffeine supplementation and FOXM1 inhibition enhance the antitumor effect of statins in neuroblastoma

Authors - Gia-Buu Tran
  • KD Validated
humanWB

Phytomedicine

Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.

Authors - Xing Yue Xu