SPRR2F Polyclonal antibody

SPRR2F Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24648-1-AP

Synonyms

small proline rich protein 2F, SPR 2F, SPRR2F

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Validation Data Gallery

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  IHC staining of mouse ovary using 24648-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24648-1-AP (SPRR2F antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 24648-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24648-1-AP (SPRR2F antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

24648-1-AP targets SPRR2F in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPRR2F fusion protein Ag20323
• Full Name: small proline-rich protein 2F
• Calculated Molecular Weight: 72 aa, 8 kDa
• GenBank Accession Number: BC156788
• Gene Symbol: SPRR2F
• Gene ID (NCBI): 6705
• RRID: AB_2879655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Cell Death Dis; Single-cell analysis reveals urothelial cell heterogeneity and regenerative cues following cyclophosphamide-induced bladder injury.; Authors - Xiaomu Cheng