Spartin, SPG20 Polyclonal antibody

• Featured Product
• KD/KO Validated

Spartin, SPG20 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 13791-1-AP

Synonyms

SPG20, TAHCCP1, SPG 20, Spartin, SPART

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Validation Data Gallery

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  WB analysis using 13791-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13791-1-AP

  WB result of Spartin, SPG20 antibody (13791-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Spartin, SPG20 transfected HeLa cells.

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  IP experiment of HeLa using 13791-1-AP

  IP result of anti-Spartin, SPG20 (IP:13791-1-AP, 4ug; Detection:13791-1-AP 1:2000) with HeLa cells lysate 2320 ug.

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  IHC staining of human liver cancer using 13791-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13791-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13791-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 13791-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Spartin, SPG20 (13791-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Product Information

13791-1-AP targets Spartin, SPG20 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Spartin, SPG20 fusion protein Ag4815
• Full Name: spastic paraplegia 20 (Troyer syndrome)
• Calculated Molecular Weight: 75 kDa, 85 kDa
• Observed Molecular Weight: 75-84 kDa
• GenBank Accession Number: BC047083
• Gene Symbol: Spartin
• Gene ID (NCBI): 23111
• RRID: AB_2195683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.

Publications

Species	Application	Title
human	WB	Ann Neurol; Developmental and degenerative features in a complicated spastic paraplegia.; Authors - Manzini M Chiara MC
human	WB	Elife; Chromosome mis-segregation and cytokinesis failure in trisomic human cells.; Authors - Joshua M Nicholson
mouse	WB,IF	Hum Mol Genet; Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.; Authors - Renvoisé Beno?t B ; KO Validated
human	WB,IF	Oncogene; SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis.; Authors - Lind G E GE
mouse	WB	J Cell Sci; Germline Deletion of Huntingtin Causes Male Infertility and Arrested Spermiogenesis in Mice.; Authors - Jinting Yan
human	WB,IF	FASEB J; A novel mutation in SPART gene causes a severe neurodevelopmental delay due to mitochondrial dysfunction with complex I impairments and altered pyruvate metabolism.; Authors - Chiara Diquigiovanni ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

beyenech (Verified Customer) (11-22-2019)

The antibody works also for Immunoprecipitation

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: IF: 1:100; WB:1:1000
• Cell Tissue Type: 293T and HeLa

Spartin, SPG20 Antibody Western Blot,Immunofluorescence, validation (IF: 1:100; WB:1:1000 dilution) in 293T and HeLa (Cat no:13791-1-AP)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C