• Featured Product
  • KD/KO Validated

SPCS1 Polyclonal antibody

SPCS1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11847-1-AP

Synonyms

SPC12, SPC1, SPase 12 kDa subunit, HSPC033



Tested Applications

Positive WB detected inHeLa cells, human skin tissue, PC-3 cells
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11847-1-AP targets SPCS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPCS1 fusion protein Ag2407
Full Name signal peptidase complex subunit 1 homolog (S. cerevisiae)
Calculated Molecular Weight 102 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC000884
Gene Symbol SPCS1
Gene ID (NCBI) 28972
RRIDAB_2195400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SPCS1 antibody 11847-1-APDownload protocol
IF protocol for SPCS1 antibody 11847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.

Authors - Rong Zhang
  • KO Validated
humanWB

PLoS Pathog

Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.

Authors - Suzuki Ryosuke R
  • KD Validated
WB

PLoS Pathog

SPCS1-Dependent E2-p7 processing determines HCV Assembly efficiency.

Authors - Nabeel Alzahrani
humanWB

Mol Cell

Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway

Authors - Iqbal Dulloo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Joao (Verified Customer) (10-09-2019)

Specific, single band (~12kDa), low background

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: 293T
SPCS1 Antibody Western Blot, validation (1:500 dilution) in 293T (Cat no:11847-1-AP)