SPAG8 Polyclonal antibody, PBS Only

SPAG8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13915-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 13915-1-AP (same clone as 13915-1-PBS)

  DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 13915-1-AP (SPAG8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 13915-1-AP (same clone as 13915-1-PBS)

  IP result of anti-SPAG8 (IP:13915-1-AP, 3ug; Detection:13915-1-AP 1:500) with mouse testis tissue lysate 6000ug. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 13915-1-AP (same clone as 13915-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13915-1-AP (SPAG8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

---

Product Information

13915-1-PBS targets SPAG8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPAG8 fusion protein Ag4910
• Full Name: sperm associated antigen 8
• Calculated Molecular Weight: 45 kDa, 54 kDa
• Observed Molecular Weight: 56-70 kDa
• GenBank Accession Number: BC019247
• Gene Symbol: SPAG8
• Gene ID (NCBI): 26206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SPAG8, also named as Sperm-associated antigen 8 or HSD-1, is a 426 amino acid protein, which is expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. SPAG8 may play a role in fertility and microtubule formation through interaction with RANBP9. SPAG8 as testis-specific protein is produced during male germ cell differentiation and functions in a germ cell type- and stage-specific manner during spermatogenesis.