SNX4 Polyclonal antibody

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  WB analysis using 11506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11506-1-AP (SNX4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11506-1-AP

  WB result of SNX4 antibody (11506-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX4 transfected A549 cells.

• 

  WB analysis using 11506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11506-1-AP (SNX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11506-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11506-1-AP (SNX4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 11506-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11506-1-AP (SNX4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

SNX4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11506-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Sorting nexin-4, sorting nexin 4

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, A431 cells, A549 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11506-1-AP targets SNX4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX4 fusion protein Ag2058
• Full Name: sorting nexin 4
• Calculated Molecular Weight: 450 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC018762
• Gene Symbol: SNX4
• Gene ID (NCBI): 8723
• RRID: AB_2286398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95219
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Recycling of autophagosomal components from autolysosomes by the recycler complex.; Authors - Chuchu Zhou