SNX20 Polyclonal antibody

SNX20 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13180-1-AP

Synonyms

SLIC 1, SLIC1, SNX20, sorting nexin 20

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Validation Data Gallery

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  WB analysis of COLO 320 using 13180-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13180-1-AP (SNX20 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13180-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13180-1-AP (SNX20 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 13180-1-AP

  IP result of anti-SNX20 (IP:13180-1-AP, 4ug; Detection:13180-1-AP 1:300) with COLO 320 cells lysate 2000ug.

• 

  IHC staining of human placenta using 13180-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 13180-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 13180-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 13180-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse thymus tissue
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human placenta tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13180-1-AP targets SNX20 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX20 fusion protein Ag3847
• Full Name: sorting nexin 20
• Calculated Molecular Weight: 316 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC027944
• Gene Symbol: SNX20
• Gene ID (NCBI): 124460
• RRID: AB_10638911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.