SNRPA Polyclonal antibody

• Featured Product
• KD/KO Validated

SNRPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (2)

Cat no : 10212-1-AP

Synonyms

U1-A, U1A, U1 snRNP A, U1 A

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Validation Data Gallery

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  WB analysis using 10212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10212-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10212-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10212-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10212-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10212-1-AP

  IP result of anti-SNRPA (IP:10212-1-AP, 4ug; Detection:10212-1-AP 1:500) with HEK-293 cells lysate 2000 ug.

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  IHC staining of human cervical cancer using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10212-1-AP (SNRPA Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10212-1-AP (SNRPA Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10212-1-AP (SNRPA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, A431 cells, HepG2 cells, mouse liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human cervical cancer tissue, human colon tissue, human liver tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

10212-1-AP targets SNRPA in WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRPA fusion protein Ag0283
• Full Name: small nuclear ribonucleoprotein polypeptide A
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC000405
• Gene Symbol: SNRPA
• Gene ID (NCBI): 6626
• RRID: AB_2239723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP A1 functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes.

Publications

Species	Application	Title
human	WB,ChIP	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB,IHC,IF	Cell Prolif; SNRPA enhances tumour cell growth in gastric cancer through modulating NGF expression.; Authors - Ning Dou ; KD Validated
canine	WB	Biochem Biophys Res Commun; Basolateral sorting of the Mg²⁺ transporter CNNM4 requires interaction with AP-1A and AP-1B.; Authors - Yusuke Hirata ; KD Validated
human	WB	Front Oncol; Designer Exosomes for Targeted Delivery of a Novel Therapeutic Cargo to Enhance Sorafenib-Mediated Ferroptosis in Hepatocellular Carcinoma; Authors - Xiaoju Li
human	WB	RNA Biol; Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.; Authors - Sikandar Azam
human	RIP	Mol Cell; Transcription elongation defects link oncogenic SF3B1 mutations to targetable alterations in chromatin landscape; Authors - Prajwal C Boddu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

changwei (Verified Customer) (02-18-2020)

It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa S3

FH

Yaroslav (Verified Customer) (01-06-2020)

Specific signal with modest sensitivity at current dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse ES cells

SNRPA Antibody Western Blot, validation (1:1000 dilution) in mouse ES cells (Cat no:10212-1-AP)