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  • KD/KO Validated

SMPDL3B Polyclonal antibody

SMPDL3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16552-1-AP

Synonyms

ASM like phosphodiesterase 3b, ASML3B, SMPDL3B



Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16552-1-AP targets SMPDL3B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMPDL3B fusion protein Ag9535
Full Name sphingomyelin phosphodiesterase, acid-like 3B
Calculated Molecular Weight373aa,42 kDa; 455aa,51 kDa
Observed Molecular Weight42 kDa, 50-65 kDa
GenBank Accession NumberBC014444
Gene Symbol SMPDL3B
Gene ID (NCBI) 27293
RRIDAB_2878276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMPDL3B is lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. SMPDL3B acts as a negative regulator of Toll-like receptor signaling.It lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP in vitro (PMID: 26095358). SMPDL3B can be detected 60-70 kDa by N-glycosylation modification (PMID: 26095358, PMID: 31217420).

Protocols

Product Specific Protocols
WB protocol for SMPDL3B antibody 16552-1-APDownload protocol
IHC protocol for SMPDL3B antibody 16552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Mol Biosci

SMPDL3B Predicts Poor Prognosis and Contributes to Development of Acute Myeloid Leukemia

Authors - Huiqing Qu
  • KO Validated
ratWB,IHC

Basic Clin Pharmacol Toxicol

PMID: 29923306

Authors - Xin Li
mouseWB

JHEP Rep

Macrophage-derived thrombospondin 1 promotes obesity-associated non-alcoholic fatty liver disease.

Authors - Taesik Gwag
human,pig,monkeyWB,IF

iScience

Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism

Authors - Huan-Huan Shen
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kamal (Verified Customer) (02-15-2024)

This antibody works well for mouse liver tissues.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Mouse liver tissue