SMOX Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SMOX Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15052-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of PC-13 using 15052-1-AP (same clone as 15052-1-PBS)

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15052-1-AP (same clone as 15052-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 15052-1-AP (same clone as 15052-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15052-1-AP (same clone as 15052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15052-1-AP (same clone as 15052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 15052-1-AP (same clone as 15052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 15052-1-AP (same clone as 15052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 15052-1-AP (same clone as 15052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 15052-1-AP (same clone as 15052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

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Product Information

15052-1-PBS targets SMOX in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMOX fusion protein Ag7035
• Full Name: spermine oxidase
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 56-65 kDa
• GenBank Accession Number: BC000669
• Gene Symbol: SMOX
• Gene ID (NCBI): 54498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SMOX(Spermine oxidase) is also named as SMO, PAO-1 and PAOh1. It belongs to the class of polyamine oxidases (PAOs).The flavoprotein SMO, which specifically oxidizes Spm, is one of the key regulatory enzymes involved in polyamine homeostasis in animal cells (PMID:14764092). It has 6 isoforms produced by alternative splicing with the MW of 61 kDa, 56 kDa, 21 kDa, 60 kDa, 43 kDa and 65 kDa, respectively.