SMOX Polyclonal antibody

• Featured Product
• KD/KO Validated

SMOX Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

33

Cat no : 15052-1-AP

Synonyms

C20orf16, PAO, PAO 1, PAOh1, Polyamine oxidase 1, SMO, SMOX, spermine oxidase

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Validation Data Gallery

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  WB analysis of PC-13 using 15052-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15052-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, PC-13 cells, MCF-7 cells, HeLa cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue, human stomach cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Product Information

15052-1-AP targets SMOX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMOX fusion protein Ag7035
• Full Name: spermine oxidase
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 56-65 kDa
• GenBank Accession Number: BC000669
• Gene Symbol: SMOX
• Gene ID (NCBI): 54498
• RRID: AB_2239689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMOX(Spermine oxidase) is also named as SMO, PAO-1 and PAOh1. It belongs to the class of polyamine oxidases (PAOs).The flavoprotein SMO, which specifically oxidizes Spm, is one of the key regulatory enzymes involved in polyamine homeostasis in animal cells (PMID:14764092). It has 6 isoforms produced by alternative splicing with the MW of 61 kDa, 56 kDa, 21 kDa, 60 kDa, 43 kDa and 65 kDa, respectively.

Publications

Species	Application	Title
human	WB	J Clin Invest; Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma.; Authors - Anna Bianchi-Smiraglia
mouse	WB	J Clin Invest; Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.; Authors - Leslie A Sandusky-Beltran
mouse	WB	EBioMedicine; Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.; Authors - Guangtian Wang
human	WB	J Invest Dermatol; The Polyamine Putrescine Promotes Human Epidermal Melanogenesis.; Authors - Aishwarya Sridharan
		Free Radic Biol Med; Adaptive responses of heart and skeletal muscle to spermine oxidase overexpression: Evaluation of a new transgenic mouse model.; Authors - Roberta Ceci
human	WB,IHC	Biomedicines; Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients.; Authors - Sooyoun Kim