SMCO4 Polyclonal antibody

SMCO4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25815-1-AP

Synonyms

C11orf75, UPF0443 protein C11orf75, Single-pass membrane and coiled-coil domain-containing protein 4, Protein FN5, FN5

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Validation Data Gallery

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  WB analysis of HEK-293 using 25815-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25815-1-AP (SMCO4 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25815-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25815-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 25815-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 25815-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25815-1-AP targets SMCO4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMCO4 fusion protein Ag22806
• Full Name: chromosome 11 open reading frame 75
• Calculated Molecular Weight: 59 aa, 7 kDa
• Observed Molecular Weight: 7 kDa
• GenBank Accession Number: BC031564
• Gene Symbol: SMCO4
• Gene ID (NCBI): 56935
• RRID: AB_2880249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMCO4, also named as C11orf75 and FN5, is a Single-pass membrane and coiled-coil domain-containing protein.