SLITRK5 Polyclonal antibody

SLITRK5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21496-1-AP

Synonyms

SLIT and NTRK like protein 5, LRRC11, Leucine-rich repeat-containing protein 11, KIAA0918, FLJ58374

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Validation Data Gallery

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  WB analysis using 21496-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21496-1-AP (SLITRK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 21496-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SLITRK5 antibody (21496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21496-1-AP targets SLITRK5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLITRK5 fusion protein Ag15880
• Full Name: SLIT and NTRK-like family, member 5
• Calculated Molecular Weight: 958 aa, 107 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC098106
• Gene Symbol: SLITRK5
• Gene ID (NCBI): 26050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLITRK5 (NTRK-like protein-5), a neuron-specific transmembrane protein, acts as a negative regulator of hedgehog signaling in osteoblasts. SLITRK5 is selectively expressed in osteoblasts and loss of SLITRK5 enhanced osteoblast differentiation in vitro and in vivo. It is a therapeutic target to enhance bone formation (PMID: 34326333). SLITRK5 deficiency impairs corticostriatal circuitry and leads to obsessive-compulsive-like behaviors in mice (PMID: 20418887).