SLC7A6 Polyclonal antibody

SLC7A6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13823-1-AP

Synonyms

KIAA0245, LAT 2, LAT3, SLC7A6, Y+L amino acid transporter 2, y+LAT 2, Y+LAT2

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Validation Data Gallery

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  WB analysis using 13823-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13823-1-AP (SLC7A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 13823-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13823-1-AP (SLC7A6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13823-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13823-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13823-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13823-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HUVEC cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

13823-1-AP targets SLC7A6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC7A6 fusion protein Ag4924
• Full Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 6
• Calculated Molecular Weight: 515 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC028216
• Gene Symbol: SLC7A6
• Gene ID (NCBI): 9057
• RRID: AB_2877978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
chicken	WB	Front Physiol; Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers; Authors - Yun Hu
chicken	WB	J Anim Sci; Zinc proteinate with moderate chelation strength enhances zinc absorption by upregulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos; Authors - Yun Hu
chicken	WB	Poult Sci; The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers; Authors - Yun Hu