SLC6A8 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SLC6A8 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20299-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 20299-1-AP (same clone as 20299-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20299-1-AP (SLC6A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 20299-1-AP (same clone as 20299-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 20299-1-AP (SLC6A8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20299-1-AP (SLC6A8 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

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Product Information

20299-1-PBS targets SLC6A8 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC6A8 fusion protein Ag14110
• Full Name: solute carrier family 6 (neurotransmitter transporter, creatine), member 8
• Calculated Molecular Weight: 635 aa, 71 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC012355
• Gene Symbol: SLC6A8
• Gene ID (NCBI): 6535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SLC6A8, also known as the sodium- and chloride-dependent creatine transporter 1 (CT1), plays a critical role in transporting creatine, a crucial molecule for energy metabolism, into cells. SLC6A8 belongs to the solute carrier family 6 (SLC6), responsible for transporting diverse molecules across cell membranes. SLC6A8 expression is highest in muscle, kidney, and other tissues with high energy demands. Mutations in SLC6A8 cause creatine transporter deficiency, an X-linked mental retardation disorder (PMID: 17465020).