ZnT6 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZnT6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13526-1-AP

Synonyms

MST103, MSTP103, SLC30A6, Zinc transporter 6, ZnT 6, ZNT6

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Validation Data Gallery

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  WB analysis of A375 using 13526-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13526-1-AP (ZnT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13526-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13526-1-AP (ZnT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A375 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below

Product Information

13526-1-AP targets ZnT6 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, chicken, Yellow catfish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZnT6 fusion protein Ag4403
• Full Name: solute carrier family 30 (zinc transporter), member 6
• Calculated Molecular Weight: 460 aa, 50 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC032525
• Gene Symbol: ZnT6
• Gene ID (NCBI): 55676
• RRID: AB_2254797
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZnT6 (encoded by SLC30A6) is a member of zinc transporters. ZnT6 is localized on the membrane of the Golgi apparatus as well as cytoplasmic vesicles. It may function in transporting the cytoplasmic zinc into the Golgi apparatus as well as the vesicular compartment. Altered expression of ZnT6 may correlate to neural diseases including Alzheimer's disease. Several isoforms of ZnT6 exist due to the alternative splicing, with MW around 48-56 kDa.

Publications

Species	Application	Title
mouse	WB	Acta Neuropathol Commun; Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.; Authors - Alexandra Grubman
human	WB	J Biol Chem; Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transport complexes in the early secretory pathway.; Authors - Fukunaka Ayako A
mouse	WB	Metallomics; Deregulation of biometal homeostasis: the missing link for neuronal ceroid lipofuscinoses?; Authors - Alexandra Grubman
chicken	WB	J Biol Chem; Detailed analyses of the crucial functions of Zn transporter proteins in alkaline phosphatase activation.; Authors - Eisuke Suzuki ; KO Validated
Yellow catfish	WB	Biochim Biophys Acta Gene Regul Mech; Transcriptional responses of four slc30a/znt family members and their roles in Zn homeostatic modulation in yellow catfish Pelteobagrus fulvidraco.; Authors - Chang-Chun Song
human, mouse	WB	J Neurosci Res; Zinc transporters ZnT3 and ZnT6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis.; Authors - Masayuki Kaneko