ZnT3 Polyclonal antibody

ZnT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 17363-1-AP

Synonyms

SLC30A3, Zinc transporter 3, ZnT 3, ZNT3

---

Validation Data Gallery

• 

  WB analysis using 17363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17363-1-AP (ZnT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 17363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17363-1-AP (ZnT3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17363-1-AP (ZnT3 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

17363-1-AP targets ZnT3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZnT3 fusion protein Ag10735
• Full Name: solute carrier family 30 (zinc transporter), member 3
• Calculated Molecular Weight: 388 aa, 42 kDa
• Observed Molecular Weight: 42 kDa, 48 kDa
• GenBank Accession Number: BC028358
• Gene Symbol: ZnT3
• Gene ID (NCBI): 7781
• RRID: AB_2239335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZnT3, encoded by SLC30A3, is a member of SLC30A family which is zinc transporter sequestrating cytosolic zinc into intracellular compartments or effluxing zinc to the extracellular space. Excessive zinc level can cause neuron apoptosis and lead to cognitive impairments. Znt3 facilitates the transport and storage of zinc in synaptic vesicles. Abnormal ZnT3 expression has been linked to some neurodegenerative disorders.

Publications

Species	Application	Title
mouse	WB	Acta Neuropathol Commun; Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.; Authors - Alexandra Grubman
	WB	J Cereb Blood Flow Metab; MicroRNA-30a regulates acute cerebral ischemia-induced blood-brain barrier damage through ZnT4/zinc pathway.; Authors - Peng Wang
mouse	IHC	Cell Biosci; Disruption of zinc transporter ZnT3 transcriptional activity and synaptic vesicular zinc in the brain of Huntington's disease transgenic mouse.; Authors - Li Niu
human	WB	Int J Mol Sci; MicroRNA-5572 Is a Novel MicroRNA-Regulating SLC30A3 in Sporadic Amyotrophic Lateral Sclerosis.; Authors - Hisaka Kurita
human, mouse	WB, IHC, IF	J Neurosci Res; Zinc transporters ZnT3 and ZnT6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis.; Authors - Masayuki Kaneko
human	WB,IF	Biochem Biophys Res Commun; Protective roles of SLC30A3 against endoplasmic reticulum stress via ERK1/2 activation.; Authors - Hisaka Kurita