SLC25A40 Polyclonal antibody

SLC25A40 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20615-1-AP

Synonyms

MCFP

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Validation Data Gallery

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  WB analysis using 20615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20615-1-AP (SLC25A40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 20615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: C2C12 cells, mouse kidney tissue, mouse liver tissue
• Positive IHC detected in: human placenta tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20615-1-AP targets SLC25A40 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A40 fusion protein Ag14669
• Full Name: solute carrier family 25, member 40
• Calculated Molecular Weight: 338 aa, 38 kDa
• Observed Molecular Weight: 30~38 kDa
• GenBank Accession Number: BC027322
• Gene Symbol: SLC25A40
• Gene ID (NCBI): 55972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A40 (solute carrier family 25, member 40) is one of the solute carrier (SLC) transporters that comprise a family of MCS transporters and uptakes GSH into mitochondria (PMID: 37407483). The SLC25A40 mRNA levels were significantly increased in both the kidneys of LPS mice and KMRC-1 cells after LPS treatment, when compared with those in the controls (PMID: 35955707).