SLC24A4 Polyclonal antibody

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• KD/KO Validated

SLC24A4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18992-1-AP

Synonyms

Solute carrier family 24 member 4, Sodium/potassium/calcium exchanger 4, NCKX4, Na(+)/K(+)/Ca(2+)-exchange protein 4

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Validation Data Gallery

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  WB analysis using 18992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 18992-1-AP

  WB result of SLC24A4 antibody (18992-1-AP, 1:1000) with si-Control and si-SLC24A4 transfected HeLa cells.

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  WB analysis of mouse brain using 18992-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 18992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 18992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, mouse heart tissue
• Positive IHC detected in: mouse brain tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

18992-1-AP targets SLC24A4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 62-90 kDa
• GenBank Accession Number: NM_153646
• Gene Symbol: SLC24A4
• Gene ID (NCBI): 123041
• RRID: AB_10596939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC24A4, also named as NCKX4, belongs to the sodium/potassium/calcium exchanger family. SLC24A4 transports 1 Ca2+ and 1 K+ in exchange for 4 Na+. Genetic variations in SLC24A4 are associated with skin/hair/eye pigmentation type 6 (SHEP6).

Publications

Species	Application	Title
mouse	IHC	Bone; Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis.; Authors - Akane Inoue
rat	WB	J Cell Physiol; Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling.; Authors - Lacruz Rodrigo S RS
mouse	WB	Calcif Tissue Int; Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.; Authors - A L J J Bronckers
human	WB	PeerJ; Identification and validation of calcium extrusion-related genes prognostic signature in colon adenocarcinoma; Authors - Mingpeng Jin
mouse	WB	Cells Tissues Organs; Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.; Authors - Hu Ping P