DMT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

90

Cat no : 20507-1-AP

Synonyms

SLC11A2, Solute carrier family 11 member 2, OK/SW-cl.20, Natural resistance-associated macrophage protein 2, DMT-1

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Validation Data Gallery

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  WB analysis using 20507-1-AP

  Various cell lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 20507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 20507-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 20507-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20507-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 20507-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse small intestine using 20507-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using DMT1 antibody (20507-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 20507-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DMT1 antibody (20507-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 20507-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DMT1 antibody (20507-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HuH-7 cells, COLO 320 cells, Caco-2 cells, rat kidney tissue, SH-SY5Y cells, Neuro-2a cells
• Positive IHC detected in: mouse brain tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse small intestine tissue, mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 83 publications below
• IHC: See 5 publications below
• IF: See 6 publications below

Product Information

20507-1-AP targets DMT1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DMT1 fusion protein Ag14314
• Full Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
• Calculated Molecular Weight: 568 aa, 62 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC002592
• Gene Symbol: DMT1
• Gene ID (NCBI): 4891
• RRID: AB_10694284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that play a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including the brain, kidney, testis, duodenum, and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

Publications

Species	Application	Title
human	WB	Blood; Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.; Authors - Floriane Petit
human	IHC	Acta Neuropathol; Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.; Authors - Till S Zimmer
mouse	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	Sci Adv; Magnetically attracting hydrogel reshapes iron metabolism for tissue repair; Authors - Yichang Xu
mouse	IF	Nat Commun; Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration.; Authors - Hirofumi Yamaguchi
rat	WB	Transl Res; Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats.; Authors - Guang Yang