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  • KD/KO Validated

SKIL Polyclonal antibody

SKIL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB,IP,IHC,IF,ELISA

Conjugate

Unconjugated

Cat no : 19218-1-AP

Synonyms

SKI like oncogene, Ski like protein, Ski related oncogene, Ski related protein, SKIL, SNO, SnoA, SnoI, SnoN



Tested Applications

Positive WB detected inA431 cells, human skeletal muscle tissue, HepG2 cells, MCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19218-1-AP targets SKIL in WB,IP,IHC,IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKIL fusion protein Ag5282
Full Name SKI-like oncogene
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC059386
Gene Symbol SKIL
Gene ID (NCBI) 6498
RRIDAB_10859824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.

Protocols

Product Specific Protocols
WB protocol for SKIL antibody 19218-1-APDownload protocol
IHC protocol for SKIL antibody 19218-1-APDownload protocol
IF protocol for SKIL antibody 19218-1-APDownload protocol
IP protocol for SKIL antibody 19218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Cell Death Differ

PIAS1 and TIF1γ collaborate to promote SnoN SUMOylation and suppression of epithelial-mesenchymal transition.

Authors - Ayan Chanda
human,mouseWB,IHC

Clin Transl Med

NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA stabilization

Authors - Shaomin Zou
mouseWB

J Med Chem

Structure-Guided Design of a Small-Molecule Activator of Sirtuin-3 that Modulates Autophagy in Triple Negative Breast Cancer.

Authors - Jin Zhang

J Neurosci

The Transcriptional Regulator SnoN Promotes the Proliferation of Cerebellar Granule Neuron Precursors in the Postnatal Mouse Brain.

Authors - Xiaoying Chen
humanWB

Mol Cell Proteomics

Quantitative ubiquitylome analysis reveals specificity of RNF111/Arkadia E3 ubiquitin ligase for its degradative substrates SKI and SKIL/SnoN in TGF-β signaling pathway

Authors - Victor Laigle
humanWB,IHC,IF

Apoptosis

SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.

Authors - Zuo-Long Wu
  • KD Validated