SKAP2 Monoclonal antibody, PBS Only

SKAP2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G3G9

Cat no : 66367-1-PBS

Synonyms

PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R

---

Validation Data Gallery

• 

  WB analysis of RAW 264.7 using 66367-1-Ig (same clone as 66367-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 66367-1-Ig (same clone as 66367-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66367-1-Ig (same clone as 66367-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66367-1-Ig (same clone as 66367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66367-1-Ig (same clone as 66367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 66367-1-Ig (same clone as 66367-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SKAP2 antibody (66367-1-Ig, Clone: 2G3G9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66367-1-PBS targets SKAP2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat, mouse samples.

• Tested Reactivity: human, pig, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SKAP2 fusion protein Ag3615
• Full Name: src kinase associated phosphoprotein 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC036044
• Gene Symbol: SKAP2
• Gene ID (NCBI): 8935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.