SIX5 Polyclonal antibody

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• KD/KO Validated

SIX5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22938-1-AP

Synonyms

Homeobox protein SIX5, DMAHP, DM locus-associated homeodomain protein

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Validation Data Gallery

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  WB analysis using 22938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22938-1-AP (SIX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22938-1-AP

  WB result of SIX5 antibody (22938-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIX5 transfected HeLa cells.

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  IP experiment of Y79 using 22938-1-AP

  IP result of anti-SIX5 (IP:22938-1-AP, 4ug; Detection:22938-1-AP 1:300) with Y79 cells lysate 2000ug.

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  IHC staining of human kidney using 22938-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22938-1-AP (SIX5 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Y79 cells
• Positive IP detected in: Y79 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

22938-1-AP targets SIX5 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIX5 fusion protein Ag18993
• Full Name: SIX homeobox 5
• Calculated Molecular Weight: 739 aa, 75 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC156983
• Gene Symbol: SIX5
• Gene ID (NCBI): 147912
• RRID: AB_2879186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Immunity; An atlas of cells in the human tonsil; Authors - Ramon Massoni-Badosa
mouse	WB	iScience; RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis; Authors - Hannah J Wiedner