SIX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIX2 Polyclonal Antibody for IF, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IP, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

235

Reviews (1)

Cat no : 11562-1-AP

Synonyms

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

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Validation Data Gallery

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  WB analysis using 11562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11562-1-AP

  IP result of anti-SIX2(IP:11562-1-AP, 4ug; Detection:11562-1-AP 1:1000) with HEK-293 cells lysate 1320 ug.

• 

  IF Staining of mouse embryonic kidney using 11562-1-AP

  IF result of SIX2 antibody (11562-1-AP, 1:200) with mouse embryonic kidney rudiment dissected at E13.5 and cultured for 2 days by Dr. Aleksandra Rak-Raszewska. SIX2 positive cells (red) incondense metanephric mesenchyme surrounding the ureteric bud tip.

• 

  IF Staining of heritable frontonasal dysplasia and renal hypoplas using 11562-1-AP

  IF result from Fogelgren B, PMID:18570229, "Misexpression of Six2 is associated with heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice.".

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Tested Applications

• Positive IP detected in: HEK-293 cells
• Positive IF detected in: mouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 35 publications below
• IF: See 195 publications below
• IP: See 3 publications below
• ChIP: See 5 publications below

Product Information

11562-1-AP targets SIX2 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, Alligator, canine, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIX2 fusion protein Ag2124
• Full Name: SIX homeobox 2
• Calculated molecular weight: 291 aa, 32 kDa
• Observed molecular weight: 32-37 kDa
• GenBank accession number: BC024033
• Gene symbol: SIX2
• Gene ID (NCBI): 10736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.

Publications

Species	Application	Title
human	IF	Nature; Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.; Authors - Minoru Takasato
human	IF	Nat Biotechnol; Tubuloids derived from human adult kidney and urine for personalized disease modeling.; Authors - Frans Schutgens
human	IF	Nat Biotechnol; Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D.; Authors - Ravian L van Ineveld
mouse	IF	Cell; In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.; Authors - Hsin-Kai Liao
human	IF	Cell; Tridimensional Visualization and Analysis of Early Human Development.; Authors - Morgane Belle
mouse	IF	Cell Stem Cell; Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells.; Authors - Atsuhiro Taguchi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nicolas (Verified Customer) (09-17-2020)

I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: E18.5 kidney

SIX2 Antibody Immunofluorescence validation (1/200 dilution) in E18.5 kidney (Cat no:11562-1-AP)

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