SIX2 Polyclonal antibody

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  WB analysis using 11562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11562-1-AP

  IP result of anti-SIX2 (IP:11562-1-AP, 4ug; Detection:11562-1-AP 1:1000) with HEK-293 cells lysate 1320 ug.

• Featured Product
• KD/KO Validated

The SIX2 polyclonal antibody (11562-1-AP) is the highest cited SIX2 antibody in the market with 270 citations.

Cat No. 11562-1-AP

Publications(270)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IP, ChIP, ELISA

Synonyms

SIX homeobox 2, Sine oculis homeobox homolog 2, Homeobox protein SIX2

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Tested Applications

• Positive WB detected in: HEK-293 cells, L02 cells, PC-3 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 32 publications below
• IP: See 3 publications below
• ChIP: See 5 publications below

Product Information

11562-1-AP targets SIX2 in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine, alligator
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2124

  Product name: Recombinant human SIX2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-291 aa of BC024033

  Sequence: MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHAKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELTEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNSNSHNPLNGSGKSVLGSSEDEKTPSGTPDHSSSSPALLLSPPPPGLPSLHSLGHPPGPSAVPVPVPGGGGADPLQHHHGLQDSILNPMSANLVDLGS

• Full Name: SIX homeobox 2
• Calculated Molecular Weight: 291 aa, 32 kDa
• Observed Molecular Weight: 32-37 kDa
• GenBank Accession Number: BC024033
• Gene Symbol: SIX2
• Gene ID (NCBI): 10736
• RRID: AB_2189084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NPC8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Nature; Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.; Authors - Minoru Takasato
human		Nat Biotechnol; Tubuloids derived from human adult kidney and urine for personalized disease modeling.; Authors - Frans Schutgens
human		Nat Biotechnol; Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D.; Authors - Ravian L van Ineveld
mouse		Cell; In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.; Authors - Hsin-Kai Liao
human		Cell; Tridimensional Visualization and Analysis of Early Human Development.; Authors - Morgane Belle
mouse		Cell Stem Cell; Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells.; Authors - Atsuhiro Taguchi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Prakash (Verified Customer) (10-28-2025)

works good in western blot.

• Applications: Western Blot
• Cell Tissue Type: HEK-293 cells

FH

Prakash (Verified Customer) (10-28-2025)

works good in western blot

• Applications: Western Blot
• Cell Tissue Type: HEK-293 cells

FH

Nicolas (Verified Customer) (09-17-2020)

I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: E18.5 kidney

SIX2 Antibody Immunofluorescence validation (1/200 dilution) in E18.5 kidney (Cat no:11562-1-AP)