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- KD/KO Validated
SIX2 Polyclonal antibody
SIX2 Polyclonal Antibody for IF, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (5)
Applications
WB, IP, IF, ChIP, ELISA
Conjugate
Unconjugated
235
Cat no : 11562-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IP detected in | HEK-293 cells |
Positive IF detected in | mouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice |
Recommended dilution
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 27 publications below |
IHC | See 35 publications below |
IF | See 195 publications below |
IP | See 3 publications below |
ChIP | See 5 publications below |
Product Information
11562-1-AP targets SIX2 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, Alligator, canine, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SIX2 fusion protein Ag2124 |
Full Name | SIX homeobox 2 |
Calculated molecular weight | 291 aa, 32 kDa |
Observed molecular weight | 32-37 kDa |
GenBank accession number | BC024033 |
Gene symbol | SIX2 |
Gene ID (NCBI) | 10736 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SIX2 antibody 11562-1-AP | Download protocol |
IP protocol for SIX2 antibody 11562-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. | ||
Nat Biotechnol Tubuloids derived from human adult kidney and urine for personalized disease modeling. | ||
Nat Biotechnol Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D. | ||
Cell In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. | ||
Cell Stem Cell Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Nicolas (Verified Customer) (09-17-2020) | I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)
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