Tested Applications
| Positive WB detected in | HEK-293 cells, L02 cells, PC-3 cells |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 30 publications below |
| IP | See 3 publications below |
| ChIP | See 5 publications below |
Product Information
11562-1-AP targets SIX2 in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, rabbit, canine, alligator |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SIX2 fusion protein Ag2124 Predict reactive species |
| Full Name | SIX homeobox 2 |
| Calculated Molecular Weight | 291 aa, 32 kDa |
| Observed Molecular Weight | 32-37 kDa |
| GenBank Accession Number | BC024033 |
| Gene Symbol | SIX2 |
| Gene ID (NCBI) | 10736 |
| RRID | AB_2189084 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPC8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIX2 antibody 11562-1-AP | Download protocol |
| IP protocol for SIX2 antibody 11562-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. | ||
Nat Biotechnol Tubuloids derived from human adult kidney and urine for personalized disease modeling. | ||
Nat Biotechnol Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D. | ||
Cell In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. | ||
Cell Stem Cell Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Nicolas (Verified Customer) (09-17-2020) | I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)
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