SIRT1 Recombinant antibody

• Featured Product
• KD/KO Validated

SIRT1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2F11

Cat no : 82790-4-RR

Synonyms

NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286, EC:2.3.1.-

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Validation Data Gallery

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  WB analysis using 82790-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82790-4-RR (SIRT1 antibody) at dilution of 1:4700 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82790-4-RR

  WB result of SIRT1 antibody (82790-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRT1 transfected HeLa cells.

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  IP experiment of HEK-293 using 82790-4-RR

  IP result of anti-SIRT1 (IP:82790-4-RR, 4ug; Detection:82790-4-RR 1:1000) with HEK-293 cells lysate 1360 ug.

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  IF Staining of HepG2 using 82790-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIRT1 antibody (82790-4-RR, Clone: 2F11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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  IF Staining of HepG2 using 82790-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIRT1 antibody (82790-4-RR, Clone: 2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 82790-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82790-4-RR against Human SIRT1 were performed. The affinity constant is 4.90 nM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82790-4-RR targets SIRT1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SIRT1 fusion protein Ag3808
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Calculated Molecular Weight: 747 aa, 82 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC012499
• Gene Symbol: SIRT1
• Gene ID (NCBI): 23411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttles between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.