SIGIRR Polyclonal antibody

SIGIRR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27828-1-AP

Synonyms

Toll/interleukin-1 receptor 8, Toll/interleukin 1 receptor 8, TIR8, Single immunoglobulin domain-containing IL1R-related protein, Single Ig IL-1R-related molecule

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Validation Data Gallery

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  WB analysis of mouse lung using 27828-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27828-1-AP (SIGIRR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• ELISA: See 1 publications below

Product Information

27828-1-AP targets SIGIRR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIGIRR fusion protein Ag25850
• Full Name: single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC025953
• Gene Symbol: SIGIRR
• Gene ID (NCBI): 59307
• RRID: AB_2880984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIGIRR (also known as TIR8) is a member of the toll-like receptor (TLR)/interleukin 1 receptor (IL-1R) superfamily, with a single immunoglobulin extracellular domain and a TIR (Toll/IL-1R) intracellular domain. SIGIRR functions as a negative regulator for IL-1 and LPS signaling, through its interaction with the TLR4 and IL-1R complex. SIGIRR is an important modulator of intestinal epithelial homeostasis and a key regulator of mucosal immunity, maintaining microbial tolerance of the intestinal epithelial layer (PMID: 17398123). The molecular weight of the glycosylated SIGIRR was found to be in the range 50-80 kDa (PMID: 10346978; 17495864).

Publications

Species	Application	Title
pig	WB	Viruses; Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage.; Authors - Shunli Yang
human	WB	Cell Death Dis; IL-37b alleviates endothelial cell apoptosis and inflammation in Kawasaki disease through IL-1R8 pathway.; Authors - Chang Jia
human,mouse	WB,ELISA	Ital J Pediatr; SIGIRR-caspase-8 signaling mediates endothelial apoptosis in Kawasaki disease; Authors - Zhengwang Wen