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  • KD/KO Validated

Sur-8 Polyclonal antibody

Sur-8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17561-1-AP

Synonyms

SHOC2, Protein sur-8 homolog, Protein sur 8 homolog, Protein soc-2 homolog, Protein soc 2 homolog



Tested Applications

Positive WB detected inMCF-7 cells, mouse brain tissue, rat testis tissue, Jurkat cells, U-251 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17561-1-AP targets Sur-8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sur-8 fusion protein Ag11754
Full Name soc-2 suppressor of clear homolog (C. elegans)
Calculated Molecular Weight 582 aa, 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC050445
Gene Symbol SHOC2/Sur-8
Gene ID (NCBI) 8036
RRIDAB_2188463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Sur-8 antibody 17561-1-APDownload protocol
IHC protocol for Sur-8 antibody 17561-1-APDownload protocol
IF protocol for Sur-8 antibody 17561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP,WB

J Clin Invest

Desmoglein-1/Erbin interaction suppresses ERK activation to support epidermal differentiation.

Authors - Harmon Robert M RM
mouseWB

EMBO J

Phosphorylation of Pkp1 by RIPK4 regulates epidermal differentiation and skin tumorigenesis.

Authors - Philbert Lee
monkeyWB, IP

J Cell Sci

Spatial control of Shoc2 scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5.

Authors - Eun Ryoung Jang
humanWB, IP

Mol Cell Biol

HUWE1 Is a Molecular Link Controlling RAF-1 Activity Supported by the Shoc2 Scaffold.

Authors - Eun Ryoung Jang
humanIHC

Mol Cancer Res

SHOC2 Is a Critical Modulator of Sensitivity to EGFR-TKIs in Non-Small Cell Lung Cancer Cells.

Authors - Hideki Terai
  • KO Validated
humanWB,IP

J Cell Sci

The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.

Authors - Patricia Wilson
  • KD Validated