SHOC2/Sur-8 Polyclonal antibody

• Featured Product
• KD/KO Validated

SHOC2/Sur-8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 17561-1-AP

Synonyms

SHOC2, Sur-8, Protein sur-8 homolog, Protein sur 8 homolog, Protein soc-2 homolog

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Validation Data Gallery

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  WB analysis using 17561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17561-1-AP (Sur-8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 17561-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17561-1-AP (Sur-8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17561-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17561-1-AP (Sur-8 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17561-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17561-1-AP (Sur-8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17561-1-AP (Sur-8 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17561-1-AP (Sur-8 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 17561-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Sur-8 antibody (17561-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 17561-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Sur-8 antibody (17561-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse brain tissue, rat testis tissue, Jurkat cells, U-251 cells
• Positive IHC detected in: human renal cell carcinoma tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IP: See 4 publications below

Product Information

17561-1-AP targets SHOC2/Sur-8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHOC2/Sur-8 fusion protein Ag11754
• Full Name: soc-2 suppressor of clear homolog (C. elegans)
• Calculated Molecular Weight: 582 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC050445
• Gene Symbol: SHOC2/Sur-8
• Gene ID (NCBI): 8036
• RRID: AB_2188463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP,WB	J Clin Invest; Desmoglein-1/Erbin interaction suppresses ERK activation to support epidermal differentiation.; Authors - Harmon Robert M RM
mouse	WB	EMBO J; Phosphorylation of Pkp1 by RIPK4 regulates epidermal differentiation and skin tumorigenesis.; Authors - Philbert Lee
monkey	WB, IP	J Cell Sci; Spatial control of Shoc2 scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5.; Authors - Eun Ryoung Jang
human	WB, IP	Mol Cell Biol; HUWE1 Is a Molecular Link Controlling RAF-1 Activity Supported by the Shoc2 Scaffold.; Authors - Eun Ryoung Jang
human	IHC	Mol Cancer Res; SHOC2 Is a Critical Modulator of Sensitivity to EGFR-TKIs in Non-Small Cell Lung Cancer Cells.; Authors - Hideki Terai ; KO Validated
human	WB,IP	J Cell Sci; The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.; Authors - Patricia Wilson ; KD Validated