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- KD/KO Validated
Sur-8 Polyclonal antibody
Sur-8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 17561-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, mouse brain tissue, rat testis tissue, Jurkat cells, U-251 cells |
Positive IHC detected in | human renal cell carcinoma tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 7 publications below |
IHC | See 1 publications below |
IP | See 4 publications below |
Product Information
17561-1-AP targets Sur-8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Sur-8 fusion protein Ag11754 |
Full Name | soc-2 suppressor of clear homolog (C. elegans) |
Calculated Molecular Weight | 582 aa, 65 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC050445 |
Gene Symbol | SHOC2/Sur-8 |
Gene ID (NCBI) | 8036 |
RRID | AB_2188463 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for Sur-8 antibody 17561-1-AP | Download protocol |
IHC protocol for Sur-8 antibody 17561-1-AP | Download protocol |
IF protocol for Sur-8 antibody 17561-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Clin Invest Desmoglein-1/Erbin interaction suppresses ERK activation to support epidermal differentiation. | ||
EMBO J Phosphorylation of Pkp1 by RIPK4 regulates epidermal differentiation and skin tumorigenesis. | ||
J Cell Sci Spatial control of Shoc2 scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5. | ||
Mol Cell Biol HUWE1 Is a Molecular Link Controlling RAF-1 Activity Supported by the Shoc2 Scaffold. | ||
Mol Cancer Res SHOC2 Is a Critical Modulator of Sensitivity to EGFR-TKIs in Non-Small Cell Lung Cancer Cells.
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J Cell Sci The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.
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