Mouse Shh Recombinant antibody, PBS Only

Shh Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240057E7

Cat no : 83280-1-PBS

Synonyms

M100081, Hxl3, Hx, Hhg1, Dsh

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Validation Data Gallery

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  WB analysis using 83280-1-RR (same clone as 83280-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 83280-1-RR (same clone as 83280-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Shh antibody (83280-1-RR, Clone: 240057E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83280-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83280-1-RR against Mouse SHH were performed. The affinity constant is below 1 pM.

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Product Information

83280-1-PBS targets Shh in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: sonic hedgehog
• Calculated Molecular Weight: 48KD
• Observed Molecular Weight: 19-50 kDa
• GenBank Accession Number: NM_009170.3
• Gene Symbol: Shh
• Gene ID (NCBI): 20423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.