SFXN1 Monoclonal antibody, PBS Only (Capture)

SFXN1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D9F1

Cat no : 68024-1-PBS

Synonyms

Tricarboxylate carrier protein, TCC, Sideroflexin-1, sideroflexin 1, 2D9F1

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Validation Data Gallery

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  WB analysis using 68024-1-Ig (same clone as 68024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68024-1-Ig (SFXN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68024-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68024-1-Ig (same clone as 68024-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SFXN1 antibody (68024-1-Ig, Clone: 2D9F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68024-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50630-2

  Cytometric bead array standard curve of MP50630-2, SFXN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68024-1-PBS. Detection antibody: 68024-4-PBS. Standard:Ag2985. Range: 0.781-100 ng/mL.

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Product Information

68024-1-PBS targets SFXN1 as part of a matched antibody pair:

MP50630-2: 68024-1-PBS capture and 68024-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SFXN1 fusion protein Ag2985
• Full Name: sideroflexin 1
• Calculated Molecular Weight: 35.6 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC020517
• Gene Symbol: SFXN1
• Gene ID (NCBI): 94081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.