SFRS6 Polyclonal antibody

SFRS6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11772-1-AP

Synonyms

B52, Pre mRNA splicing factor SRP55, SFRS6, SRP55, SRSF6



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

11772-1-AP targets SFRS6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS6 fusion protein Ag2412
Full Name splicing factor, arginine/serine-rich 6
Calculated Molecular Weight 344 aa, 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC006832
Gene Symbol SFRS6
Gene ID (NCBI) 6431
RRIDAB_2185212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SR proteins have a characteristic C-terminal Ser/Arg-rich repeat (RS domain) of variable length and constitute a family of highly conserved nuclear phosphoproteins that can function as both essential and alternative pre-mRNA splicing factors [PMID:7556075]. SRFS6 has a pivotal role in the TF exon 5 definition, an event essential for flTF biosynthesis. Besides, up- or down regulation of SRSF6 alters the splicing of several tumor suppressors and oncogenes to generate the oncogenic isoforms and reduce the tumor suppressive isoforms [PMID:23132731].

Protocols

Product Specific Protocols
WB protocol for SFRS6 antibody 11772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.

Authors - Yuhan Chen
mouseWB

Gene

Intron retention as a new pre-symptomatic marker of aging and its recovery to the normal state by a traditional Japanese multi-herbal medicine.

Authors - Norihiro Okada
mouseWB

Data Brief

Data of RNA-seq transcriptomes of liver, bone, heart, kidney and blood in klotho mice at a pre-symptomatic state and the effect of a traditional Japanese multi-herbal medicine, juzentaihoto.

Authors - Norihiro Okada

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jingwen (Verified Customer) (04-28-2021)

The beautiful staining signal showed up inside the nucleus.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: Human cancer cell line