SF3A1 Polyclonal antibody

SF3A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15858-1-AP


PRP21, PRPF21, SAP 114, SAP114, SF3A1, SF3A120, Splicing factor 3A subunit 1

Tested Applications

Positive WB detected inHL-60 cells, mouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, K-562 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15858-1-AP targets SF3A1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF3A1 fusion protein Ag8196
Full Name splicing factor 3a, subunit 1, 120kDa
Calculated Molecular Weight 793 aa, 89 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC007684
Gene Symbol SF3A1
Gene ID (NCBI) 10291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )


Product Specific Protocols
WB protocol for SF3A1 antibody 15858-1-APDownload protocol
IHC protocol for SF3A1 antibody 15858-1-APDownload protocol
IF protocol for SF3A1 antibody 15858-1-APDownload protocol
IP protocol for SF3A1 antibody 15858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Rep

SF3B1 Association with Chromatin Determines Splicing Outcomes.

Authors - Nir Kfir

Front Pharmacol

LXR agonist inhibits inflammation through regulating MyD88 mRNA alternative splicing

Authors - Ni Li


Integrative identification of non-coding regulatory regions driving metastatic prostate cancer

Authors - Brian J Woo