SETD3 Polyclonal antibody, PBS Only

SETD3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28692-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 28692-1-AP (same clone as 28692-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28692-1-AP (SETD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 28692-1-AP (same clone as 28692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 28692-1-AP (same clone as 28692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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Product Information

28692-1-PBS targets SETD3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SETD3 fusion protein Ag30017
• Full Name: SET domain containing 3
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_032233
• Gene Symbol: SETD3
• Gene ID (NCBI): 84193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SETD3 (SET domain protein 3), a ubiquitously expressed protein, is epigenetically up-regulated in response to muscle loading and hypertrophy. The SETD3 mRNA is ubiquitously expressed at a similar basal level in most examined tissues, with the noticeable exception of the skeletal muscle, kidneys, and testes (PMID: 34685411). SETD3 deficient female mice have severely decreased litter sizes due to primary maternal dystocia that is refractory to ecbolic induction agents (PMID: 30626964).