SEPHS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SEPHS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16635-1-AP

Synonyms

SELD, Selenide, water dikinase 1, Selenium donor protein 1, Selenophosphate synthase 1, selenophosphate synthetase 1, SEPHS1, SPS, SPS1

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Validation Data Gallery

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  WB analysis using 16635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16635-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16635-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16635-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 16635-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, mouse liver tissue, T-47D cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16635-1-AP targets SEPHS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEPHS1 fusion protein Ag9978
• Full Name: selenophosphate synthetase 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC000941
• Gene Symbol: SEPHS1
• Gene ID (NCBI): 22929
• RRID: AB_10603472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Selenophosphate synthetase 1 (SEPHS1) has been implicated in the maintenance of redox homeostasis in chondrocytes and its deficiency plays a causal role in the progression of osteoarthritis (PMID: 36202216). SEPHS1 downregulation in human umbilical vein cells (HUVECs) led to cell death rather than the inhibition of cell proliferation (PMID: 33622411).

Publications

Species	Application	Title
	WB	Biomaterials; H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence; Authors - Fan Yang
human	WB,IHC	Exp Hematol Oncol; SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.; Authors - Shu Yang ; KD Validated