SEMA7A Polyclonal antibody

SEMA7A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18070-1-AP

Synonyms

CD108, CDw108, H SEMA K1, H Sema L, JMH, JMH blood group antigen, Sema K1, Sema L, SEMA7A, SEMAK1, SEMAL, Semaphorin 7A, Semaphorin K1, Semaphorin L

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Validation Data Gallery

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  WB analysis using 18070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18070-1-AP (SEMA7A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 18070-1-AP

  IP result of anti-SEMA7A (IP:18070-1-AP, 4ug; Detection:18070-1-AP 1:500) with human placenta tissue lysate 2600 ug.

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse brain tissue
• Positive IP detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

18070-1-AP targets SEMA7A in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEMA7A fusion protein Ag12707
• Full Name: semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• Calculated Molecular Weight: 666 aa, 75 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC101643
• Gene Symbol: SEMA7A
• Gene ID (NCBI): 8482
• RRID: AB_2878489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane‐associated glycosylphosphatidylinositol (GPI)‐linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, Sema7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor (PMID: 31179640). SEMA7A has 2 isoforms with molecular weights of 73 and 75 kDa, respectively.

Publications

Species	Application	Title
human	WB	J Clin Invest; EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer; Authors - Xiaochao Tan
mouse	WB	Mol Metab; Sema7A protects against high-fat diet-induced obesity and hepatic steatosis by regulating adipo/lipogenesis; Authors - Qiongyu Lu