Validation Data Gallery
|Positive WB detected in||HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue, human heart tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, mouse liver tissue, mouse heart tissue, mouse brain tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14865-1-AP targets SDHA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SDHA fusion protein Ag6677|
|Full Name||succinate dehydrogenase complex, subunit A, flavoprotein (Fp)|
|Calculated molecular weight||73 kDa|
|Observed molecular weight||62-73 kDa|
|GenBank accession number||BC001380|
|Gene ID (NCBI)||6389|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.
|Product Specific Protocols|
|WB protocol for SDHA antibody 14865-1-AP||Download protocol|
|IHC protocol for SDHA antibody 14865-1-AP||Download protocol|
|IF protocol for SDHA antibody 14865-1-AP||Download protocol|
|IP protocol for SDHA antibody 14865-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jessica (Verified Customer) (08-19-2019)
This antibody worked great for western blots on whole cell lysates and purified mitochondria!