SDHA Polyclonal antibody

• Featured Product
• KD/KO Validated

SDHA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

103

Reviews (1)

Cat no : 14865-1-AP

Synonyms

SDH 2, PPGL5, PGL5, MC2DN1, FP

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Validation Data Gallery

• 

  WB analysis using 14865-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14865-1-AP (SDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14865-1-AP

  IP result of anti-SDHA (IP:14865-1-AP, 4ug; Detection:14865-1-AP 1:20000) with HEK-293 cells lysate 960 ug.

• 

  IHC staining of human liver cancer using 14865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14865-1-AP (SDHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14865-1-AP (SDHA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14865-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SDHA antibody (14865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 97 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Product Information

14865-1-AP targets SDHA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDHA fusion protein Ag6677
• Full Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 62-73 kDa
• GenBank Accession Number: BC001380
• Gene Symbol: SDHA
• Gene ID (NCBI): 6389
• RRID: AB_11182164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Publications

Species	Application	Title
human	WB	Science; Fumarate is a terminal electron acceptor in the mammalian electron transport chain.; Authors - Jessica B Spinelli ; KO Validated
mouse	WB	Cell Metab; The requirement for pyruvate dehydrogenase in leukemogenesis depends on cell lineage.; Authors - Sojeong Jun
human	WB	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	Mol Cell; Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery; Authors - Jasmin Adriana Schafer
mouse	WB	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jessica (Verified Customer) (08-19-2019)

This antibody worked great for western blots on whole cell lysates and purified mitochondria!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Osteosarcoma cell line

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