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SCRIB Polyclonal antibody, PBS Only

SCRIB Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27083-1-PBS

Synonyms

Protein scribble homolog, Protein LAP4, LAP4, KIAA0147, hScrib

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCRIB Polyclonal antibody (27083-1-AP)

    WB analysis using 27083-1-AP (same clone as 27083-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27083-1-AP (SCRIB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SCRIB Polyclonal antibody (27083-1-AP)

    IHC staining of human ovary cancer using 27083-1-AP (same clone as 27083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27083-1-AP (SCRIB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27083-1-PBS in a different storage buffer formulation.

Product Information

27083-1-PBS targets SCRIB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCRIB fusion protein Ag25795
Full Name scribbled homolog (Drosophila)
Calculated Molecular Weight 1630 aa, 175 kDa
Observed Molecular Weight 260 kDa
GenBank Accession NumberBC036905
Gene Symbol SCRIB
Gene ID (NCBI) 23513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SCRIB encodes a large, 260-kDa cytoplasmic scaffolding protein that comprises a large leucine-rich repeat (LRR) region and 4 PDZ domains that regulate protein-protein interactions. In mammals, cell polarity is established and maintained by at least 3 protein modules (Scrib, Crumbs, and Par). The apical (Crumbs and Par) and basolateral (Scrib) modules function in a mutually antagonistic relationship to regulate various polarization processes such as apical-basal polarity, planar cell polarity, asymmetric cell division and migration. The loss of SCRIB in malignant tumors suggest that it has potential to be a tumor suppressor. Alteration of polarity through deletion, downregulation, overexpression, and mislocalization can induce structural and functional alteration of cells that might be related to tumorigenesis. Mislocalization of SCRIB is involved in epithelial-to-mesenchymal transition (EMT) by inducing loss of E-cadherin. Deregulated SCRIB expression induces tumorigenesis in breast and liver. (PMID: 28460446, PMID: 32564009)