SCML1 Polyclonal antibody

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• KD/KO Validated

SCML1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30257-1-AP

Synonyms

SCML1

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Validation Data Gallery

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  WB analysis using 30257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30257-1-AP (SCML1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 30257-1-AP

  WB result of SCML1 antibody (30257-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML1 transfected HepG2 cells.

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30257-1-AP targets SCML1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCML1 fusion protein Ag33003
• Full Name: sex comb on midleg-like 1 (Drosophila)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC026159
• Gene Symbol: SCML1
• Gene ID (NCBI): 6322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.