ChromoTek SARS-CoV-2 Spike Recombinant VHH [NM1224]

Validation Data Gallery

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  BLI binding kinetics of SARS-CoV-2 Spike Recombinant VHH [NM1224] to RBD. Biotinylated RBD was immobilized on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of SARS-CoV-2 Spike Recombinant VHH [NM1224] (ChromoTek sc-NM1224).

Recombinant monovalent Nanobody against the Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein

Cat No. sc-NM1224

Specificity

Virus

Applications

BLI, Multiplex ACE2 competition assay

Type

Nanobody

Conjugate

Unconjugated

Synonyms

SARS-CoV-2, SARS-CoV-2 Spike, Spike

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Product Information

Recombinant monovalent Nanobody against the Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein

• Specificity: Virus
• Host: Alpaca, recombinantly produced
• Applications: BLI, Multiplex ACE2 competition assay
• Class: Recombinant
• Type: Nanobody / VHH, monovalent
• Clonality: Monoclonal
• Clone No.: NM1224 [Wagner et al. EMBO Rep. 2021 May 5;22(5):e52325. PMID: 33904225]
• Immunogen: RBD of SARS-CoV-2 Spike protein
• Conjugate: Unconjugated
• Epitope: Amino acids 350-361 and 443-452
• Full Name: SARS-CoV-2 Spike Protein
• Form: Liquid
• Affinity (KD): 6.1 nM
• Calculated MW: 25 kDa
• GenBank Accession Number: NC_045512
• Gene Symbol: COVID-19 S Protein
• GeneID (NCBI): 43740568
• Positive Controls: BLI: biotinylated RBD and SARS-CoV-2 Spike Recombinant VHH
• Cited Applications: BLI, Multiplex ACE2 competition assay ; Note: Not recommended for Western blotting
• Purification Method: Recombinant expression, affinity purification by IMAC. ; Note: SARS-CoV-2 Spike Recombinant VHH carries a C-terminal Myc-tag and His-tag.
• RRID: AB_2892250
• Storage Buffer: PBS, 0.09% sodium azide ; Safety datasheet (SDS): sodium azide
• Storage Condition: Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles.
• Stability: Stable for 1 year at -20°C/-4°F after shipment.
• Shipment: Shipped at ambient temperature.
• Size: 20 µg; 100 µg

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