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SARS-CoV-2 Spike Recombinant antibody

SARS-CoV-2 Spike Recombinant Antibody for ELISA, Neutralization

Host / Isotype

Human / IgG1

Reactivity

Virus

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

AM001414

Cat no : 91361-PTG

Synonyms

SARS-CoV-2, SARS-CoV-2 Spike, Spike

Validation Data Gallery

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  • Neutralization experiment of SARS-CoV-2 Spike using SARS-CoV-2 Spike Recombinant antibody (91361-PTG)

    Neutralization experiment of SARS-CoV-2 Spike using 91361-PTG

    SARS-CoV-2 Spike Antibody (clone AM001414) tested by Neutralization.Viral neutralization assays were performed with pseudotyped virus carrying a luciferase reporter gene and bearing the SARS-CoV-2 S1 spike glycoprotein. A549 lung epithelial target cells expressing the ACE2 receptor were incubated with virus and a graded dose of SARS-CoV-2 Spike Antibody (clone AM001414). Luciferase signal, indicative of cellular infection and viral gene expression, was measured. Viral neutralization by SARS-CoV-2 antibodies inhibits viral entry and, by extension, virus associated luciferase signal in manner proportional to antibody dose. Data provided by Active Motif®. .

  • ELISA experiment of SARS-CoV-2 Spike RBD protein using SARS-CoV-2 Spike Recombinant antibody (91361-PTG)

    ELISA experiment of SARS-CoV-2 Spike RBD protein using 91361-PTG

    SARS-CoV-2 Spike Antibody (clone AM001414) tested by ELISA.SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 µg/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (clone AM009105). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. Data provided by Active Motif®. .

Tested Applications

Positive Neutralization detected inpseudotyped virus
Positive ELISA detected inSARS-CoV-2 Spike RBD protein

Recommended dilution

ApplicationDilution
Sample dependent. To be determined by the end user.

Note: Proteintech is partnering with Active Motif to bring these cutting-edge, patient derived antibodies to our customers during this important time for COVID-19 research.

Product Information

91361-PTG targets SARS-CoV-2 Spike in ELISA, Neutralization applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Host / Isotype Human / IgG1
Class Recombinant
Type Antibody
Immunogen Virus
Full Name SARS-CoV-2 Spike Protein
Calculated Molecular Weight 141 kDa
GenBank Accession NumberNC_045512
Gene Symbol SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_2882954
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A Chromatography
Storage Buffer 140 mM Hepes, pH 7.5, 70 mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies. This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.