SAR1A Polyclonal antibody

• Featured Product
• KD/KO Validated

SAR1A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22291-1-AP

Synonyms

SARA, SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase

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Validation Data Gallery

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  WB analysis using 22291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22291-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22291-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 22291-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 22291-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22291-1-AP (SAR1A Antibody) at dilution of 1:50.

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  IHC staining of mouse skeletal muscle using 22291-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22291-1-AP (SAR1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22291-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SAR1A antibody (22291-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A431 using 22291-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SAR1A antibody (22291-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HT-1080 cells, HepG2 cells, mouse kidney tissue, HEK-293T cells, HeLa cells
• Positive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• CoIP: See 1 publications below

Product Information

22291-1-AP targets SAR1A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SAR1 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: NM_020150
• Gene Symbol: SAR1A
• Gene ID (NCBI): 56681
• RRID: AB_2879062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). The antibody is specific to SAR1A.

Publications

Species	Application	Title
human	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen ; KO Validated
human	CoIP	J Cell Sci; A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.; Authors - Jiaxin Sun ; KD Validated
rat	WB	Ann Transl Med; Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.; Authors - Kun Wang
human	WB	Epigenomics; Methylation of TMEM176A, a key ERK signaling regulator, is a novel synthetic lethality marker of ATM inhibitors in human lung cancer.; Authors - Hongxia Li
rat	WB	Cell Death Differ; C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport; Authors - Yachen Wu
human	WB	J Cell Biol; Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells; Authors - Miharu Maeda