SAMD9L Polyclonal antibody

• Featured Product
• KD/KO Validated

SAMD9L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 25173-1-AP

Synonyms

C7orf6, DRIF2, KIAA2005, SAMD9L, UEF, UEF1

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Validation Data Gallery

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  WB analysis using 25173-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 25173-1-AP (SAMD9L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25173-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25173-1-AP (SAMD9L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse spleen using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 25173-1-AP (SAMD9L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of 3T3-L1 using 25173-1-AP

  Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using SAMD9L antibody (25173-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of COLO 320 using 25173-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 25173-1-AP (SAMD9L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: rat kidney tissue, COLO 320 cells
• Positive IHC detected in: human breast cancer tissue, mouse spleen tissue, human brain tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: COLO 320 cells, 3T3-L1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

25173-1-AP targets SAMD9L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAMD9L fusion protein Ag18541
• Full Name: sterile alpha motif domain containing 9-like
• Calculated Molecular Weight: 1584 aa, 185 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC127118
• Gene Symbol: SAMD9L
• Gene ID (NCBI): 219285
• RRID: AB_2879940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sterile alpha motif domain-containing protein 9-like(SAMD9L), also named as C7orf6, DRIF2, KIAA2005 and UEF, is a cytoplasmic protein that acts as a tumor suppressor but also plays a key role in cell proliferation and the innate immune response to viral infection. SAMD9L protein is an endosome fusion facilitator which causes myeloid malignancies in mice similar to human diseases with monosomy 7. The SAMD9L protein also appears to play an important role in the brain, particularly the part of the brain that coordinates movement (the cerebellum). SAMD9L has two isoforms with MW 185 kDa and 47 kDa.

Publications

Species	Application	Title
human	WB	Nat Med; Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes.; Authors - Sushree S Sahoo
human	WB	PLoS Pathog; A paralogous pair of mammalian host restriction factors form a critical host barrier against poxvirus infection.; Authors - Xiangzhi Meng ; KO Validated
human	IHC	Immunobiology; Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations; Authors - Tiantian Cai
human	WB,IF	Brain Commun; New spinocerebellar ataxia subtype caused by SAMD9L mutation triggering mitochondrial dysregulation (SCA49).; Authors - Marc Corral-Juan
human	WB	Exp Cell Res; Viral host range factors antagonize pathogenic SAMD9 and SAMD9L variants; Authors - Stine Gahr
human	IHC	J Inflamm Res; Integrated Analysis Identifies Upregulated SAMD9L as a Potential Biomarker Correlating with the Severity of Primary Sjögren's Syndrome; Authors - Donghai Zhou