Validation Data Gallery
|Positive WB detected in||human kidney tissue, A549 cells, mouse kidney tissue, mouse lung tissue, human brain tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human brain tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13033-1-AP targets SACM1L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SACM1L fusion protein Ag3682|
|Full Name||SAC1 suppressor of actin mutations 1-like (yeast)|
|Calculated molecular weight||587 aa, 67 kDa|
|Observed molecular weight||60-67 kDa|
|GenBank accession number||BC016559|
|Gene ID (NCBI)||22908|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SACM1L(Suppressor of actin mutations 1-like protein) is also named KIAA0851. It is a phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P and has low activity towards PtdIns(3,5)P2.
|Product Specific Protocols|
|WB protocol for SACM1L antibody 13033-1-AP||Download protocol|
|IHC protocol for SACM1L antibody 13033-1-AP||Download protocol|
|IF protocol for SACM1L antibody 13033-1-AP||Download protocol|
|IP protocol for SACM1L antibody 13033-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity
Cell Host Microbe
Global mapping of Salmonella enterica-host protein-protein interactions during infection.
An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.
PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes.
Proc Natl Acad Sci U S A
Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.
J Cell Biol
Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking.