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- KD/KO Validated
S100A9 Polyclonal antibody
S100A9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 26992-1-AP
Validation Data Gallery
|Positive WB detected in
|HL-60 cells, MCF-7 cells, THP-1 cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human liver cancer tissue, mouse spleen tissue, human tonsillitis tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HeLa cells, A549 cells, HepG2 cells
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
26992-1-AP targets S100A9 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|S100A9 fusion protein Ag25764
|S100 calcium binding protein A9
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.
|Product Specific Protocols
|WB protocol for S100A9 antibody 26992-1-AP
|IHC protocol for S100A9 antibody 26992-1-AP
|IF protocol for S100A9 antibody 26992-1-AP
|IP protocol for S100A9 antibody 26992-1-AP
|Click here to view our Standard Protocols
Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.
Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt.
Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation.
Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells.
Acta Physiol (Oxf)
Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.
Mol Cell Proteomics
Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury