S100A9 Polyclonal antibody

• Featured Product
• KD/KO Validated

S100A9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14226-1-AP

Synonyms

60B8AG, CAGB, Calgranulin B, Calprotectin L1H subunit, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP 14, MRP14, NIF, P14, Protein S100 A9, S100A9

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Validation Data Gallery

• 

  WB analysis of mouse spleen using 14226-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14226-1-AP

  WB result of S100A9 antibody (14226-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A9 transfected MCF-7 cells.

• 

  WB analysis of human heart using 14226-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 14226-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14226-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 14226-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14226-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14226-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14226-1-AP (S100A9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14226-1-AP (S100A9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14226-1-AP (S100A9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14226-1-AP (S100A9 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, human blood, human heart tissue, MCF-7 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human breast cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Product Information

14226-1-AP targets S100A9 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A9 fusion protein Ag5465
• Full Name: S100 calcium binding protein A9
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 14 kDa, 28 kDa
• GenBank Accession Number: BC047681
• Gene Symbol: S100A9
• Gene ID (NCBI): 6280
• RRID: AB_2254232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Publications

Species	Application	Title
human	IHC	Cell Metab; QDPR deficiency drives immune suppression in pancreatic cancer; Authors - Ji Liu
human,mouse	IHC,IF	Int J Cancer; S100 calcium-binding protein A9 from tumor-associated macrophage enhances cancer stem cell-like properties of hepatocellular carcinoma.; Authors - Ran Wei
human,mouse	WB,IHC	Cell Biosci; TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling.; Authors - Qiang Liu ; KD Validated
rat	IF	Int J Med Sci; Single-cell RNA Sequencing Uncovered the Involvement of an Endothelial Subset in Neutrophil Recruitment in Chemically Induced Rat Pulmonary Inflammation.; Authors - Hong Huang
human	WB,IF	J Oncol; Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma; Authors - Shiyu Zeng ; KD Validated
human	IHC	Histol Histopathol; Histopathological assessment of calcification and inflammation of calcific aortic valves from patients with and without diabetes mellitus.; Authors - Josephin Mosch

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