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  • KD/KO Validated

S100A10 Polyclonal antibody

S100A10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11250-1-AP

Synonyms

Calpactin-1 light chain, Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG



Tested Applications

Positive WB detected inA431 cells, HeLa cells, HT-29 cells, human lung tissue, HaCaT cells
Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue, human gliomas tissue, human normal colon, human pancreas cancer tissue, human prostate hyperplasia tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11250-1-AP targets S100A10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A10 fusion protein Ag1779
Full Name S100 calcium binding protein A10
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC015973
Gene Symbol S100A10
Gene ID (NCBI) 6281
RRIDAB_2269906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Protocols

Product Specific Protocols
WB protocol for S100A10 antibody 11250-1-APDownload protocol
IHC protocol for S100A10 antibody 11250-1-APDownload protocol
IF protocol for S100A10 antibody 11250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.

Authors - Yunwei Lou
ratIF

JCI Insight

Fabry disease Schwann cells release p11 to induce sensory neuron hyperactivity

Authors - Tyler B Waltz
mouseWB,IP

Diabetes

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Authors - Hua Qu
  • KD Validated
humanIF

J Nanobiotechnology

Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

Authors - Xiao Zhai
humanWB

J Med Chem

5α-Epoxyalantolactone Inhibits Metastasis of Triple-Negative Breast Cancer Cells by Covalently Binding a Conserved Cysteine of Annexin A2.

Authors - Mingming Wei
human,mouseIHC

Cancers (Basel)

S100A10 Has a Critical Regulatory Function in Mammary Tumor Growth and Metastasis: Insights Using MMTV-PyMT Oncomice and Clinical Patient Sample Analysis.

Authors - Alamelu G Bharadwaj
  • KO Validated