S100A10 Polyclonal antibody

• Featured Product
• KD/KO Validated

S100A10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

44

Cat no : 11250-1-AP

Synonyms

Calpactin-1 light chain, Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG

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Validation Data Gallery

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  WB analysis using 11250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11250-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11250-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human colon using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11250-1-AP (S100A10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human skin using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human skin using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 11250-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using S100A10 antibody (11250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11250-1-AP

  Immunofluorescent analysis of HeLa cells using 11250-1-AP (S100A10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HT-29 cells, human lung tissue, HaCaT cells
• Positive IHC detected in: human lung cancer tissue, human cervical cancer tissue, human gliomas tissue, human normal colon, human pancreas cancer tissue, human prostate hyperplasia tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 28 publications below
• IHC: See 7 publications below
• IF: See 20 publications below
• IP: See 3 publications below
• ELISA: See 1 publications below
• FC: See 1 publications below

Product Information

11250-1-AP targets S100A10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A10 fusion protein Ag1779
• Full Name: S100 calcium binding protein A10
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC015973
• Gene Symbol: S100A10
• Gene ID (NCBI): 6281
• RRID: AB_2269906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.; Authors - Yunwei Lou
rat	IF	JCI Insight; Fabry disease Schwann cells release p11 to induce sensory neuron hyperactivity; Authors - Tyler B Waltz
mouse	WB,IP	Diabetes; Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.; Authors - Hua Qu ; KD Validated
human	IF	J Nanobiotechnology; Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury; Authors - Xiao Zhai
human	WB	J Med Chem; 5α-Epoxyalantolactone Inhibits Metastasis of Triple-Negative Breast Cancer Cells by Covalently Binding a Conserved Cysteine of Annexin A2.; Authors - Mingming Wei
human,mouse	IHC	Cancers (Basel); S100A10 Has a Critical Regulatory Function in Mammary Tumor Growth and Metastasis: Insights Using MMTV-PyMT Oncomice and Clinical Patient Sample Analysis.; Authors - Alamelu G Bharadwaj ; KO Validated

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