CoraLite® Plus 488-conjugated S1 tag Monoclonal antibody

S1 tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A9B5

Cat no : CL488-66165

Synonyms

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Validation Data Gallery

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  WB analysis using CL488-66165

  S1 tagged protein lysate was subjected to SDS PAGE followed by western blot with CL488-66165 (S1 tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66165 targets S1 tag in WB applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: S1 tag
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S1 tag, with sequence NANNPDWDF, is derived from the hepatitis B virus preS1 region. S1 tag is widely used in various protein expression systems. This antibody can detect the over-expressed fusion proteins containing S1 epitope tag.