Red fluorescent protein Monoclonal antibody
Red fluorescent protein Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Recombinant Protein and More (2)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1E3C5
Cat no : 67378-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | ag27669 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:100000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 5 publications below |
IF | See 1 publications below |
Product Information
67378-1-Ig targets Red fluorescent protein in WB, IF, ELISA applications and shows reactivity with Recombinant Protein samples.
Tested Reactivity | Recombinant Protein |
Cited Reactivity | mouse, yeast |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Red fluorescent protein fusion protein Ag27669 |
Full Name | Red fluorescent protein |
Calculated Molecular Weight | 26 kDa |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2882625 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a mouse (IgG2b) monoclonal antibody raised against RFP (eqFP611). It can be used to detect RFPs from eqFP611 and eqFP578 lineages.
Protocols
Product Specific Protocols | |
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WB protocol for Red fluorescent protein antibody 67378-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
bioRxiv Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins | ||
EMBO Rep Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome | ||
Mol Biol Cell Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins | ||
Int J Mol Sci Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1 | ||
Autophagy The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple |