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Rat AGER/RAGE Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: rat

Detection: rat

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.156-20 ng/mL

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP00734-1

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00734-1, Rat RAGE Recombinant Matched Antibody Pair - PBS only. 83759-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1135. 83759-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

    Sandwich ELISA standard curve of MP00734-1

    Sandwich ELISA standard curve of MP00734-1, Rat RAGE Recombinant Matched Antibody Pair - PBS only. 83759-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1135. 83759-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

  • Serum of sixteen rats was measured. The rat RAGE concentration of detected samples was determined to be 2,201.2 pg/mL with a range of 1,157.8 - 3,936.9 pg/mL.

    Sample test of MP00734-1

    Serum of sixteen rats was measured. The rat RAGE concentration of detected samples was determined to be 2,201.2 pg/mL with a range of 1,157.8 - 3,936.9 pg/mL.

  • Cytometric bead array standard curve of MP00734-1, Rat AGER/RAGE Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83759-2-PBS. Detection antibody: 83759-1-PBS. Standard: Eg1135. Range: 0.156-20 ng/mL.

    Cytometric bead array standard curve of MP00734-1

    Cytometric bead array standard curve of MP00734-1, Rat AGER/RAGE Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83759-2-PBS. Detection antibody: 83759-1-PBS. Standard: Eg1135. Range: 0.156-20 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00734-1 targets AGER/RAGE in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.156-20 ng/mL (Cytometric Bead Array); 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: Rat AGER/RAGE Recombinant antibody, PBS Only (Capture/Detector) 83759-2-PBS (240706D12). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Rat AGER/RAGE Recombinant antibody, PBS Only (Capture/Detector) 83759-1-PBS (240706C1). 100 μg. Concentration 1 mgl/ml.

Alternative AGER/RAGE matched antibody pairs: MP00693-1, MP00693-2, MP00693-3, MP00734-3

Matched pair protein standard: Eg1135

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83759-2-PBS
Tested Reactivity rat
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name advanced glycosylation end product-specific receptor
Synonyms RAGE, AGER, Advanced glycosylation end product-specific receptor, 240706D12
Gene symbol Ager
UniprotQ63495
Gene ID (NCBI) 81722
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83759-1-PBS
Tested Reactivity rat
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name advanced glycosylation end product-specific receptor
Synonyms RAGE, AGER, Advanced glycosylation end product-specific receptor, 240706C1
Gene symbol Ager
UniprotQ63495
Gene ID (NCBI) 81722
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.